ytlR [2019-09-05 09:48:36]

unknown

locus: BSU_29940

pI: 10.07

mw: 35.45 kDa

protein length: 309 aa

gene length: 930 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 3,063,833 → 3,064,762

The protein

Protein family

• diacylglycerol/lipid kinase family (with [protein|88AECCE90DC0D6C92C61AB8860A31A830A8DAC42|BmrU] and [protein|943294ACA74067A8549056699E08DF5B82337976|DgkB], according to UniProt)

Structure

• [PDB|2QV7] (Staphylococcus aureus diacylglycerol kinase, 25% identity) [pubmed|18611377]

Expression and Regulation

Operons

• genes: [gene|29A38D244A07ED0973D0846B652238524F4DBC67|ytlP]-[gene|DB1491F13F8B55A360413E815E47A2B51D24395C|ytlQ]-[gene|ED4C1EA16FF96DA20F12B972CD2458FA3B49DDA5|ytlR]-[gene|7D783DD180D950A4B030BF5EA78CCE930A466A07|amyX]
  description: [pubmed|22383849]

  sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|26577401], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

Biological materials

Mutant

• MGNA-A433 (ytlR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/433 NBRP B. subtilis, Japan]
• BKE29940 (Δ[gene|ED4C1EA16FF96DA20F12B972CD2458FA3B49DDA5|ytlR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTCTCACCTGCTTAT, downstream forward: _UP4_TAAACGTGTTTGGAGGAGAG
• BKK29940 (Δ[gene|ED4C1EA16FF96DA20F12B972CD2458FA3B49DDA5|ytlR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTCTCACCTGCTTAT, downstream forward: _UP4_TAAACGTGTTTGGAGGAGAG

References

• 26577401,18611377